P01.09. Effect of Maeg-Moon-Dong-Tang on production and secretion of respiratory mucus
نویسندگان
چکیده
Methods Confluent NCI-H292 cells were pretreated for 30 min in the presence of MMT and treated with ATP (200μM) or PMA (10ng/ml) or EGF (25ng/ml) or TNF-alpha (0.2nM) for 24 hours, to assess the effect of MMT both on ATPor PMAor EGFor TNF-alpha-induced MUC5AC mucin production using enzyme-linked immunosorbent assay (ELISA) and on gene expression by the same inducers using reverse transcription-polymerase chain reaction (RT-PCR). At the same time, hypersecretion of airway mucus was induced by exposure of rats to SO2 during 3 weeks. Effect of orallyadministered MMT during 2 weeks on increase in airway epithelial mucosubstances from tracheal goblet cells of rats was assesed using histopathological analysis after staining the epithelial tissue with PAS-alcian blue. Possible cytotoxicity of MMT was assessed by investigating the potential damage of kidney and liver function by measuring serum GOT/GPT activities and serum BUN concentration of rats and body weight gain during the experiment.
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